Phosphorylation of Histone H3.3 at Serine 31 Promotes p300 Activity

Time: 1:30 pm
day: Day Two


  • H3.3-specific S31 phosphorylation stimulates activity of the acetyltransferase p300 in trans, suggesting that H3.3 acts as a nucleosomal cofactor for p300 at enhancers
  • Depletion of H3.3 from mESCs results in a substantial reduction of acetylation on histone H3 at lysine 27 (H3K27ac) at enhancers
  • Cells lacking H3.3 demonstrate reduced capacity to acetylate enhancers that are activated upon differentiation, along with reduced ability to reprogram cell fate